ROBO4 Rabbit Polyclonal Antibody for WB, ELISA - Q8WZ75

Core Product Specifications and Parameters

Biological Background: ROBO4 Function and Localization

• ROBO4 (Roundabout homolog 4), also referred to as Magic roundabout, is a transmembrane receptor of the immunoglobulin superfamily implicated in vascular biology.
• It serves as a receptor for Slit proteins, particularly SLIT2, and is involved in angiogenesis and vascular patterning.
• ROBO4 is critical for the maintenance of endothelial barrier organization and function, contributing to vascular integrity. Related references: PMID:30455415
• The protein is specifically expressed in endothelial cells, with prominent expression in endothelial and intimal cells of the ascending aorta. Related references: PMID:30455415
• Post-translational modifications include glycosylation and phosphorylation, and the protein features conserved disulfide bonds and immunoglobulin-like domains.
• Mutations in ROBO4 are linked to disease phenotypes, underscoring its role in development and endothelial homeostasis.

Experimental Guidance and Technical Tips

• The immunogen (aa 848-1007) targets the intracellular C-terminal region of ROBO4, enabling recognition of total protein levels regardless of glycosylation state; this design is well-suited for denaturing Western blot.
• In Western blot, anticipate a band around 107 kDa; use reducing conditions and appropriate molecular weight markers for validation.
• Human reactivity is confirmed; cross-reactivity with other species has not been tested. Validate in the relevant sample system if using non-human samples.
• For ELISA, the polyclonal format may allow flexible assay designs, but optimal antibody concentrations and pairing should be titrated experimentally.
• Endothelial cell lines or vascular tissue samples are recommended as positive controls due to the target's tissue specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, supporting diagnostic developers and research laboratories globally. For vascular biology and ROBO4-related investigations, we provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our streamlined procurement bridges the gap from concept to clinic, ensuring efficient access to high-quality raw materials for your assay development.