RNF20 Rabbit Polyclonal Antibody for WB, ELISA - Q5VTR2

Core Product Specifications and Parameters

Biological Background: RNF20 Function and Localization

• RNF20, also known as BRE1A or hBRE1, is an E3 ubiquitin-protein ligase that functions as a key component of the RNF20/40 complex, mediating monoubiquitination of histone H2B at lysine 120 (H2BK120ub1).
• H2BK120ub1 serves as an epigenetic tag for transcriptional activation and is a prerequisite for subsequent histone H3 methylation at lysine 4 and 79 (H3K4me and H3K79me), positioning RNF20 centrally in the histone code and gene regulation.
• The RNF20/40 complex cooperates with E2 ubiquitin-conjugating enzymes UBE2A or UBE2B to catalyze H2B ubiquitination, and is required for transcriptional activation of Hox genes.
• RNF20 localizes predominantly to the nucleus and is recruited to specific gene promoters, such as MDM2, via interaction with p53/TP53, where it acts as a transcriptional coactivator.
• In cancer cells, RNF20 mediates polyubiquitination and proteasome-dependent degradation of isoform 2 of PA2G4.
• RNF20 contains zinc-finger domains and coiled-coil regions, and its activity is regulated by post-translational modifications including acetylation and phosphorylation.
• Tissue specificity: expressed in normal brain and malignant gliomas at the protein level.
• During viral infection, RNF20 promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing expression of lytic viral genes, including the latency switch gene RTA/ORF50.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein encompassing amino acids 1-260 of human RNF20 (N-terminal region). For Western blot, consider using whole cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is approximately 114 kDa. Optimize blocking and antibody dilution for your specific system.
• Since the antibody is suitable for ELISA, it may be used to detect recombinant or native RNF20 in capture assays; validate performance with appropriate positive and negative controls.
• As a polyclonal preparation, lot-to-lot variability may exist; consider including an internal reference standard for quantitative comparisons.
• The antibody cross-reacts with human, mouse, and rat RNF20 based on sequence homology; verify reactivity in your model organism before large-scale experiments.

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