RNF10 Rabbit Polyclonal Antibody for WB, ELISA - Q8N5U6

Core Product Specifications and Parameters

Biological Background: E3 ubiquitin-protein ligase RNF10 Function and Localization

• RNF10 is an E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2 (us5) and RPS3 (us3) in response to ribosome stalling. This modification is part of the ribosome quality control (RQC) pathway, leading to recognition by RIOK3 kinase and subsequent 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Related references: PMID:34348161, PMID:34469731, PMID:39609413
• RNF10 acts in both initiation-stalled (iRQC) and elongation-stalled ribosome quality control, and is activated by ribosome subunit imbalance, amino acid starvation, or the integrated stress response (ISR) downstream of GCN2. Related references: PMID:39609413, PMID:39947182, PMID:39947183
• The ubiquitination by RNF10 is reversed by the deubiquitinase USP10, indicating a tightly regulated mechanism. Related references: PMID:34469731
• RNF10 is predominantly localized in the cytoplasm and nucleus.
• The protein contains a RING-type zinc finger domain necessary for its E3 ligase activity, and is subject to phosphorylation and alternative splicing.
• The calculated molecular weight of human RNF10 is approximately 90 kDa, consistent with the observed protein weight.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to the C-terminal region (aa 552-811), the antibody may recognize full-length RNF10 and possibly splice variants.
• For WB using whole-cell lysates, a band near 90 kDa is expected; consider validating detection in your specific sample system.
• Cross-reactivity with mouse and rat suggests conserved epitopes, but we recommend verifying compatibility with your experimental models.
• For ELISA, the antibody can be used to detect recombinant or endogenous RNF10; optimization of coating and detection conditions is advised.
• Ensure appropriate positive and negative controls to confirm specificity and avoid false positives.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined procurement bridge for IVD raw materials. This RNF10 antibody supports investigations into ubiquitin-dependent ribosome quality control and stress responses. From validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents, we offer one-stop sourcing tailored to your assay development needs.