RING1A Rabbit mAb for WB, IHC-P, ELISA - Q06587

Core Product Specifications and Parameters

Biological Background: RING1 Function and Localization

• RING1 (Really Interesting New Gene 1) is an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2A at Lys-119, a key epigenetic mark for transcriptional repression and X chromosome inactivation.
• It is an essential component of the Polycomb repressive complex 1 (PRC1), which maintains silencing of developmental genes such as Hox clusters through chromatin modification.
• RING1 may function as a modulator of RNF2/RING2, the primary E3 ligase in PRC1, rather than providing the main catalytic activity on H2A.
• Subcellularly, RING1 localizes to the nucleus and is enriched in nuclear speckles, consistent with its role in chromatin regulation.
• The protein contains a RING-type zinc finger domain and binds zinc, features typical of E3 ubiquitin ligases.
• RING1 is subject to alternative splicing and phosphorylation, as identified by proteomics, suggesting regulatory post-translational modifications.
• Keywords: Chromatin regulator, repressor, transcription regulation, ubl conjugation pathway, zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 100–200 of human RING1A (Q06587). This region may include structural domains; consider this when designing sandwich immunoassays to avoid epitope competition.
• For Western blot (WB), the predicted molecular weight is approximately 42 kDa; validate the detected band using appropriate positive and negative cell lysates.
• In immunohistochemistry (IHC-P), expect nuclear and possibly nuclear speckle staining; optimize antigen retrieval and antibody dilution for your tissue type.
• Although the antibody is raised against a human peptide, cross-reactivity with orthologs from other species should be experimentally verified before use.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material partner, CamelBio streamlines procurement from early R&D to commercial scale. This anti-RING1A monoclonal antibody supports epigenetics and cancer research applications, and we can also supply matched antibody pairs, bulk ancillary reagents, and assist with sourcing rare-target raw materials to accelerate your assay development pipeline.