RING1A Polyclonal Antibody (Rabbit) for WB, IHC-P, ELISA - Q06587

Core Product Specifications and Parameters

Biological Background: RING1A Function and Localization

• RING1A, also known as RNF1 or Polycomb complex protein RING1, is an E3 ubiquitin-protein ligase that plays a central role in histone modification and epigenetic gene regulation.
• It constitutes one of the E3 ligases mediating monoubiquitination of histone H2A at Lys-119, a modification that serves as a specific tag for transcriptional repression and is essential for X chromosome inactivation in female mammals.
• RING1A is a core component of the Polycomb repressive complex 1 (PRC1), which maintains the silenced state of key developmental regulators such as Hox genes through chromatin remodeling and heritable changes in gene expression.
• Compared to its paralog RNF2/RING2, RING1A exhibits weaker intrinsic E3 ligase activity and is thought to function primarily as a modulator or accessory factor for RNF2/RING2-mediated histone ubiquitination.
• Multiple isoforms exist due to alternative splicing, and the protein contains a RING-type zinc finger domain essential for its ubiquitin ligase function, along with motifs for metal binding and chromatin regulation.
• RING1A localizes to the nucleus and is enriched in nuclear speckles, consistent with its role in transcription regulation and chromatin organization.
• It is subject to phosphorylation and has been identified in proteomics studies, indicating dynamic post-translational control of its activity within the nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 207-406, a region spanning part of the C-terminal domain; thus the antibody may recognize full-length RING1A and potentially some splice variants, but validation on relevant samples is recommended.
• For Western blot (WB), a band at approximately 42 kDa is expected; consider using nuclear extracts or whole-cell lysates from human, mouse, or rat cell lines as positive controls, and optimize primary antibody dilution.
• For immunohistochemistry (IHC-P), validate the antibody on paraffin-embedded tissue sections known to express RING1A, applying appropriate antigen retrieval methods and testing a range of dilutions to achieve specific nuclear staining.
• In ELISA, the polyclonal nature allows flexible use as a capture or detection reagent; however, pairing with a monoclonal antibody may improve specificity in sandwich formats—batch variability should be assessed during assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to IVD raw materials, technical support, and consulting—from concept to clinical application. For epigenetics and chromatin research, including Polycomb group proteins like RING1A, we provide ready-to-ship polyclonal antibodies, customized monoclonal development, validated antibody pairs, and bulk ancillary reagents. Whether you need single vials or entire production lots, our sourcing network ensures timely delivery of rare and standard targets to streamline your assay development and manufacturing pipelines.