RHOU Rabbit pAb - Q7L0Q8

Core Product Specifications and Parameters

Biological Background: RHOU Function and Localization

• RHOU, also known as CDC42-like GTPase 1, WRCH-1, or ARHU, is a member of the Rho family of small GTPases that share similarity with CDC42.
• It binds to and activates the serine/threonine-protein kinase PAK1, linking it to downstream signaling pathways involved in cytoskeletal dynamics. Related references: PMID:11459829
• RHOU plays a role in the regulation of cell morphology, cytoskeletal organization, and focal adhesion assembly during cell migration. Related references: PMID:11459829 PMID:17620058 PMID:18086875
• The protein stimulates quiescent cells to reenter the cell cycle, suggesting a role in cell proliferation control. Related references: PMID:11459829
• RHOU has no detectable GTPase activity; instead, its high intrinsic guanine nucleotide exchange activity indicates it is constitutively GTP-bound, enabling continuous activation of effectors. Related references: PMID:16472646
• The protein localizes to the cell membrane, Golgi apparatus membrane, focal adhesions, and podosomes, positioning it at key sites for cell adhesion and migration.
• RHOU is ubiquitously expressed in human tissues, with high levels observed in stomach, small intestine, brain, skeletal muscle, and placenta.
• Keywords from UniProt highlight its involvement in alternative splicing, isopeptide bond formation, lipoprotein modifications, and ubiquitin conjugation, indicating complex post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 40–100 of human RHOU; verify antibody specificity by testing in the relevant sample system, preferably with a peptide competition assay or lysates with overexpressed/tagged protein.
• For western blotting, consider starting with a 1:500–1:2000 dilution range, optimizing based on signal-to-noise ratio in your experimental setup. Use appropriate blocking buffers (e.g., 5% non-fat milk or BSA) to reduce background.
• For ELISA, titrate the antibody against the immunizing peptide or recombinant protein to determine optimal coating and detection conditions.
• Since the antibody cross-reacts with mouse, validate its reactivity and specificity in mouse cell or tissue lysates if cross-species experiments are planned.
• Due to the lack of detectable GTPase activity in RHOU, experiments assessing GTPase activity may not be applicable; focus on expression or localization studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to deliver high-quality IVD raw materials and technical support. This anti-RHOU rabbit polyclonal antibody is suited for research into cell morphology, migration, and cytoskeletal organization. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your workflow from concept to clinic.