RHOF Rabbit pAb for WB and ELISA - Q9HBH0

Core Product Specifications and Parameters

Biological Background: RHOF Function and Localization

• RHOF (also known as Rif or ARHF) is a member of the Rho family of small GTPases that cycles between an active GTP-bound and inactive GDP-bound state.
• Active RHOF stimulates the formation of thin, actin-rich surface projections known as filopodia, contributing to dynamic reorganization of the actin cytoskeleton.
• It functions cooperatively with CDC42 and Rac to generate additional actin-based structures, thereby increasing morphological diversity.
• The protein localizes to the cell membrane, cytoplasm, and cytoskeleton, consistent with its role in membrane-associated signaling.
• RHOF is subject to post-translational modifications including prenylation (a lipoprotein modification), acetylation, and methylation, which regulate its subcellular targeting and activity.
• Alternative splicing generates multiple isoforms, suggesting tissue- or context-specific regulation.
• As a GTP-binding protein, it requires magnesium as a cofactor for nucleotide binding and hydrolysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 40–120 of human RHOF; this sequence is conserved in mouse, supporting cross-reactivity.
• For Western blot (WB), a band around 24 kDa is expected; use appropriate positive control lysates from human or mouse cells.
• For ELISA, optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio.
• Because RHOF cycles between GDP/GTP-bound states, consider using GTPγS or GDP treatment to stabilize active or inactive conformations in functional assays.

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CamelBio supplies a broad portfolio of IVD raw materials and sourcing services, including optimized polyclonal antibodies like this anti-RHOF pAb for cytoskeleton research. Whether you need validated antibody pairs, bulk ancillary reagents, or customized rare-target sourcing, our one-stop platform supports your workflow from early R&D to clinical applications.