RFC2 Rabbit Polyclonal Antibody for WB and ELISA - P35250

Core Product Specifications and Parameters

Biological Background: RFC2 Function and Localization

• RFC2 is a subunit of the replication factor C (RFC) complex, essential for the elongation of primed DNA templates by DNA polymerases delta and epsilon; the RFC complex facilitates ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. Related references:
  PMID:9488738
• RFC2 binds ATP, which is required for the loading of PCNA (By similarity).
• RFC2 localizes to the nucleus, consistent with its role in DNA replication.
• Post-translational modifications of RFC2 include acetylation and phosphorylation; it has been identified by direct protein sequencing and proteomics.
• The RFC2 gene undergoes alternative splicing, producing multiple transcript variants.
• RFC2 is associated with Williams-Beuren syndrome, a genetic disorder.
• The calculated molecular weight of RFC2 is approximately 39 kDa, consistent with observations.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 38-297 of human RFC2, covering a central region of the protein; this design may help detect multiple isoforms resulting from alternative splicing.
• For Western blot (WB), a band around 39 kDa is expected; titrate the antibody and validate in your specific sample matrix to confirm specificity.
• In ELISA applications, optimize coating and detection conditions to suit your assay format; cross-reactivity with mouse RFC2 supports testing in murine models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs and recombinant proteins for DNA replication and cancer research. For your RFC2 studies, we provide this rabbit polyclonal antibody alongside bulk ancillary reagents and sourcing support for rare targets. Partner with us to streamline your assay development from concept to clinic.