Rev-Erbα/NR1D1 Rabbit pAb - P20393

Core Product Specifications and Parameters

Biological Background: Rev-Erbα/NR1D1 Function and Localization

• Rev-Erbα (NR1D1) is a transcriptional repressor and key component of the circadian clock, directly repressing core clock genes including BMAL1, CLOCK, and CRY1.
• Its repressive activity is heme-dependent; heme binding stimulates interaction with the NCOR1/HDAC3 corepressor complex to enhance transcriptional repression.
• Regulates diverse metabolic pathways, including lipid and bile acid metabolism, gluconeogenesis, adipogenesis, and macrophage inflammatory responses.
• Can also act as a transcriptional activator in certain contexts, such as activation of STAR in ovarian granulosa cells and GJA1 in collaboration with SP1.
• Binds DNA as either a monomer to RevRE elements or a homodimer to RevDR-2 elements, and functions as a competitive repressor of RORA.
• Widely expressed with high levels in liver, adipose tissue, skeletal muscle, and brain; expression oscillates in a circadian manner.
• Subcellular localization includes nucleus, cytoplasm, dendrites, and dendritic spines.
• Belongs to the nuclear receptor superfamily; contains zinc-finger domains and undergoes acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 280–360) spans part of the ligand-binding domain; consider suitability for detecting both monomeric and dimeric forms of Rev-Erbα.
• For Western blot, a band around 67 kDa is expected; validate in relevant cell or tissue lysates from human, mouse, or rat.
• For IHC-P and IF/ICC, optimize fixation and permeabilization conditions to preserve antigenicity.
• As a polyclonal antibody, lot-to-lot variability may occur; batch-specific validation is recommended for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting research and diagnostic development in circadian biology, metabolic disorders, and inflammation. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies like Rev-Erbα/NR1D1, bulk ancillary reagents, and specialized sourcing for rare targets. Partner with us to streamline your journey from concept to clinic.