REL Rabbit pAb for WB, ELISA - Q04864

Core Product Specifications and Parameters

Biological Background: REL Function and Localization

• Proto-oncogene c-Rel is a member of the NF-kappa-B/Rel family of transcription factors.
• It plays a role in differentiation and lymphopoiesis, contributing to immune and inflammatory responses.
• c-Rel forms homo- or heterodimers with other Rel proteins (e.g., RELA, RELB, NFKB1, NFKB2) to bind kappa-B DNA sites and regulate gene expression.
• The RELA/p65-c-Rel heterodimer acts as a transcriptional activator.
• NF-kappa-B activity is controlled by post-translational modifications (e.g., phosphorylation, acetylation) and subcellular compartmentalization; inactive complexes are sequestered in the cytoplasm by I-kappa-B inhibitors.
• Upon activation, c-Rel translocates to the nucleus to modulate target gene transcription.
• Key features include acetylation, alternative splicing, DNA-binding, nuclear localization, and phosphorylation, underscoring its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-300), which contains Rel homology domain sequences; consider this when interpreting cross-reactivity in related family members.
• For WB, a band at ~69 kDa is expected in human, mouse, or rat lysates; validate detection in the appropriate biological context.
• This polyclonal antibody may recognize both native and denatured epitopes; for ELISA, assess performance with the specific antigen format.
• Optimize antibody dilution and blocking conditions according to the experimental system; the recommended applications are WB and ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research labs with a comprehensive supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like REL, which are central to NF-kB signaling and immune regulation, we can source rare specificities and provide reliable procurement from concept to clinic. Contact us to streamline your research and development sourcing.