RBX1 Rabbit pAb for WB and ELISA - P62877

Core Product Specifications and Parameters

Biological Background: RBX1 Function and Localization

• RBX1 (also known as ROC1, RNF75) is a core component of multiple cullin-RING E3 ubiquitin ligase (CRL) complexes, which target proteins for ubiquitination and proteasomal degradation, regulating cell cycle progression, signal transduction, and transcription.
  Related references: PMID:10230407, PMID:10579999, PMID:11961546
• Together with ARIH1, CRL complexes mediate sequential ubiquitination, where ARIH1 adds the first ubiquitin to substrates.
  Related references: PMID:27565346
• As part of the CSA complex, RBX1 ubiquitinates RNA polymerase II (POLR2A) at Lys-1268, a key step in transcription-coupled nucleotide excision repair (TC-NER).
  Related references: PMID:32355176, PMID:34526721
• RBX1 is a core component of the Cul7-RING(FBXW8) complex, which targets specific proteins for degradation.
  Related references: PMID:35982156
• It also forms a Cul9-RING ligase complex that mono-ubiquitinates p53/TP53.
  Related references: PMID:38605244
• RBX1 recruits the E2 enzyme CDC34 to the CRL complex and may stimulate its autoubiquitination; it also promotes neddylation of cullins via UBE2M.
• Involved in ubiquitination of KEAP1, ENC1, and KLHL41, and in concert with ATF2 and CUL3, promotes degradation of KAT5, modulating ATM activation.
• The protein localizes to both cytoplasm and nucleus, is widely expressed, and contains a zinc-finger domain essential for its E3 ligase activity; it is subject to acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human RBX1 (NP_055063.1). For WB, consider using whole-cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is ~12 kDa. Use appropriate blocking and detection conditions.
• For ELISA, the antibody may be used for detecting RBX1 in serum or purified protein assays; validate with appropriate positive and negative controls.
• Given RBX1’s central role in ubiquitination, consider using proteasome inhibitors (e.g., MG132) to stabilize ubiquitinated substrates in your experimental setup.
• Optimize antibody dilution for each application and sample type; for cross-reactivity with mouse and rat, verify species-specific sequence homology in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier, offering a full range of antibodies, antigens, and ancillary reagents for diagnostic and research applications. Our anti-RBX1 polyclonal antibody supports studies in ubiquitin-proteasome pathway, DNA repair, and cancer biology. Explore our catalog for validated antibody pairs, bulk conjugate formats, and customized sourcing solutions to streamline your assay development.