RBP2 Rabbit pAb - P50120

Core Product Specifications and Parameters

Biological Background: RBP2 Function and Localization

• RBP2 (retinol-binding protein 2), also known as cellular retinol-binding protein II (CRBP-II), is an intracellular lipid-binding protein that specifically binds and transports retinol (vitamin A).
• It belongs to the fatty acid-binding protein family and plays a critical role in the intracellular trafficking of retinol, influencing vision, cellular differentiation, and metabolism.
• Subcellularly, RBP2 is primarily localized in the cytoplasm, consistent with its function as an intracellular carrier for hydrophobic ligands.
• Tissue expression analysis reveals high levels of RBP2 in adult small intestine, with lower expression detected in fetal kidney, underscoring its role in dietary retinol absorption and metabolism.
• The protein is classified under keywords including retinol-binding, transport, vitamin A metabolism, and cytoplasm; its three-dimensional structure has been experimentally determined.

Experimental Guidance and Technical Tips

• The immunogen used for generating this antibody corresponds to the N-terminal region (amino acids 1-134) of human RBP2, which may be exposed in the native protein and suitable for Western blot detection.
• For WB analysis, a band at approximately 16 kDa is expected; recommend using appropriate molecular weight markers and validating with relevant cell or tissue lysates (e.g., small intestine).
• In IF/ICC, anticipate cytoplasmic staining; fixation and permeabilization conditions should be optimized for the target cell type.
• Cross-reactivity with mouse and rat RBP2 is indicated; however, sequence alignment and further validation may be necessary for other species.
• ELISA applications are supported; pairing with a suitable detection antibody and optimizing coating conditions can enhance assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This RBP2 polyclonal antibody is ideal for retinol transport and metabolism studies. For bulk orders, customized sourcing of small molecules, or technical consultation, contact CamelBio's expert team to accelerate your project from concept to clinic.