RBMX/hnRNP G Rabbit mAb for WB, IHC-P, ELISA - P38159

Core Product Specifications and Parameters

Biological Background: RBMX/hnRNP G Function and Localization

• RBMX (RNA-binding motif protein, X chromosome), also known as hnRNP G, is a 42 kDa glycoprotein (p43) encoded by the RBMX gene located on the X chromosome.
• It functions as an RNA-binding protein that regulates pre- and post-transcriptional processes, including alternative splicing, transcription, and mRNA processing.
• RBMX/hnRNP G plays a role in tumor suppression by binding to and stimulating transcription from the TXNIP gene promoter, and in lipid metabolism via activation of the SREBF1 promoter when associated with SAFB.
• It is a component of the supraspliceosome complex and regulates alternative splice site selection, either activating or suppressing exon inclusion; for example, it promotes exon 7 inclusion in SMN2 and represses MAPT/Tau exon 10 splicing.
• Subcellular localization is predominantly nuclear, consistent with its role in transcription and splicing regulation.
• Tissue-specifically, RBMX protein is strongly expressed in oral keratinocytes but weakly detected in oral squamous cell carcinomas, suggesting a potential link to tumor suppression.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitin-like conjugation, which may regulate its activity and interactions.
• It preferentially binds single-stranded 5′-CC[A/C]-rich RNA motifs as a homodimer and also interacts non-specifically with pre-mRNAs.
• Additionally, RBMX is involved in cytoplasmic TNFR1 trafficking, promoting IL-1β-mediated proteolytic cleavage and exosome-like vesicle release.

Experimental Guidance and Technical Tips

• The immunogen is designed against a sequence within amino acids 292-391, which corresponds to a central region of RBMX/hnRNP G. This region may be conserved across species, supporting the observed cross-reactivity with human, mouse, and rat.
• For Western blot (WB), a band at approximately 42 kDa is expected. Due to post-translational modifications, additional bands or slight shifts may be observed; consider validation with appropriate controls.
• In immunohistochemistry (IHC-P), nuclear staining is anticipated based on the subcellular localization of RBMX. Optimize antigen retrieval conditions and validate staining patterns in known positive and negative tissues.
• For ELISA, the antibody may be used for direct or indirect detection; confirm specificity by testing with the immunogen peptide or recombinant protein.
• When applying this antibody to new applications or species not listed, independent verification is recommended to ensure compatibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a reliable, one-stop sourcing solution for high-quality IVD raw materials. This RBMX/hnRNP G monoclonal antibody supports research into RNA processing and tumor suppression, and CamelBio offers a broad portfolio of validated antibodies and ancillary reagents to streamline your assay development. Contact us for customized bulk supply and technical support to accelerate your project from concept to clinic.