RBM20 Rabbit Polyclonal Antibody for WB, ELISA - Q5T481

Core Product Specifications and Parameters

Biological Background: RBM20 Function and Localization

• RBM20 is an RNA-binding protein that acts as a regulator of mRNA splicing of key structural proteins in cardiac development, such as TTN, CACNA1C, CAMK2D, and PDLIM5/ENH. Related references: PMID:22466703, PMID:24960161, PMID:26604136
• It represses splicing by binding the 5'UCUU-3' motif in intronic sequences, causing exon exclusion in targets. Related references: PMID:24960161, PMID:30948719, PMID:34732726
• RBM20-mediated exon skipping is hormone-dependent and essential for TTN isoform transitions in muscle, also generating circular RNAs from TTN transcripts. Related references: PMID:27531932, PMID:30948719, PMID:34732726
• With RBM24, it promotes short PDLIM5/ENH isoforms in cardiomyocytes.
• It localizes to the nucleus, cytoplasm, and cytoplasmic ribonucleoprotein granules.
• Mainly expressed in heart, with additional expression in skeletal muscle, ovary, small intestine, and colon. Related references: PMID:19712804, PMID:23886709
• Classified under cardiomyopathy, disease variant, zinc-finger, and mRNA processing keywords, emphasizing its clinical significance in cardiac disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 820–980 of human RBM20, a C-terminal region; consider this when designing competition or blocking experiments.
• For Western blot, validate antibody specificity using heart tissue lysates as a positive control, and include negative controls (e.g., non-expressing cell lines) to assess background.
• Given its polyclonal nature, lot-to-lot consistency should be verified for critical quantitative assays.
• In ELISA, the antibody can be employed for titer determination or antigen capture; titrate to determine optimal working concentration.
• Note that RBM20 undergoes tissue-specific isoform transitions; sample preparation may influence detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive supply chain for IVD raw materials, including validated antibodies like this anti-RBM20 polyclonal antibody. Whether you need antibody pairs, bulk ancillary reagents, or rare target sourcing for cardiovascular disease research, CamelBio streamlines procurement from discovery to clinical application.