RBFOX2 Rabbit Polyclonal Antibody for WB, ELISA - O43251

Core Product Specifications and Parameters

Biological Background: RBFOX2 Function and Localization

• RBFOX2, also known as Fox-1 homolog B, RNA binding protein fox-1 homolog 2, RBM9, HRNBP2, and RTA, is a member of the RBFOX family of splicing regulators.
• It functions as an RNA-binding protein that regulates alternative splicing by binding to the 5'-UGCAUGU-3' motif, preventing U2AF2 recruitment to the 3' splice site.
• RBFOX2 regulates alternative splicing of tissue-specific exons and differentially spliced exons during erythropoiesis.
• It acts as a coregulatory factor of estrogen receptor alpha (ER-alpha), modulating transcriptional activity in conjunction with other RNA-binding proteins.
• Together with RBPMS and MBNL1/2, RBFOX2 activates alternative splicing events in vascular smooth muscle cells, as shown in recent studies. Related references: PMID:37548402
• Subcellularly, it is localized to both the nucleus and cytoplasm, consistent with its roles in transcription, splicing, and mRNA processing.
• Post-translational modifications include phosphorylation and methylation, which may regulate its activity and interactions.
• The protein has a molecular weight of approximately 41.4 kDa (calculated) and contains RNA recognition motifs.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight of RBFOX2 is ~41 kDa; however, isoforms may produce bands at different sizes due to alternative splicing.
• When performing ELISA, consider using a standard curve with known concentrations of recombinant RBFOX2 protein to estimate target abundance.
• The antibody is raised against the N-terminal region (aa 1-110), which is conserved across isoforms; verify specificity using isoform-specific controls or knockdown samples.
• Cross-reactivity is reported for human; for other species, check sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD antibodies, including this RBFOX2 polyclonal antibody, suitable for alternative splicing research and diagnostic assay development. Our integrated sourcing platform offers validated antibody pairs, bulk ancillary reagents, and technical support to streamline your workflow from concept to clinic.