Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for Flow Cytometry - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The H2-Eb1 gene encodes the I-E beta chain (SWISS: P18468) of mouse H-2 class II histocompatibility antigens, a core component of MHC class II molecules.
• MHC class II molecules are heterodimeric transmembrane glycoproteins critical for adaptive immunity; they present extracellular peptide antigens to CD4+ T cells.
• Subcellular localization is exclusively at the membrane, featuring a signal peptide and a transmembrane helix as indicated in UniProt.
• Post-translational modifications include N-linked glycosylation (Glycoprotein keyword) and ubiquitin conjugation (Ubl conjugation keyword).
• The antibody cross-reacts with multiple MHC class II chains: I-A beta (P06342, P14435) and I-E beta (P18468), reflecting broad allele recognition.
• Structural features such as a disulfide bond and the Ig-like C1-type domain are noted, consistent with typical MHC class II architecture.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , making it ready-to-use for flow cytometry without secondary detection reagents.
• Titrate the antibody (e.g., 1:50–1:200) with relevant positive and negative controls to determine optimal staining concentration.
• Use mouse splenocytes or MHC class II-expressing cell lines as positive controls; include Fc receptor blocking to reduce background.
• Avoid repeated freeze-thaw cycles; store protected from light to preserve fluorophore stability.
• The monoclonal nature and recombinant immunogen ensure high batch-to-batch consistency and specificity for mouse I-A/I-E epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for diagnostics and life science research. This anti-MHC class II antibody supports flow cytometry-based immune profiling, and our team assists with sourcing rare-target reagents, technical consultation, and scalable supply solutions from concept to clinic.