Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for Flow Cytometry - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II Function and Localization

• The target protein, H-2 class II histocompatibility antigen I-A beta chain (encoded by H2-Eb1 in mice), is a transmembrane glycoprotein and a core component of MHC class II molecules.
• MHC class II molecules are heterodimers of an alpha and a beta chain; they present processed extracellular antigen peptides to CD4+ helper T cells, initiating adaptive immune responses.
• The antibody cross-reacts with multiple allelic variants of I-A (beta chains H2-Ab1 and H2-Aa) and I-E (H2-Eb1 and H2-Eb2), providing broad recognition of mouse MHC class II.
• Subcellular localization is primarily at the cell membrane, consistent with its role in antigen presentation on the surface of professional antigen-presenting cells such as dendritic cells, B cells, and macrophages.
• The beta chain contains key structural features including a signal peptide, an immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic tail, as inferred from sequence analysis.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are important for proper folding and stability.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen was designed to generate a rat monoclonal antibody targeting multiple mouse MHC class II alleles (I-A and I-E), likely recognizing a conserved epitope.
• As this antibody is directly conjugated to , it is ready-to-use for flow cytometry. Consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Include appropriate isotype control and Fc receptor blocking steps to minimize non-specific binding, especially when working with primary cell samples rich in Fc receptor-expressing cells.
• Validate staining specificity using MHC class II-deficient mouse strains or blocking with excess unlabeled antibody, if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research entities in procuring high-quality IVD raw materials. For immunology-focused projects, we offer validated monoclonal antibodies like this anti-MHC Class II clone, along with bulk ancillary reagents and rare target sourcing. Whether you need antibody pairs for assay development or optimized reagents for flow cytometry, our one-stop platform streamlines your supply chain from discovery to clinical application.