Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for FC - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The target protein is encoded by the H2-Eb1 gene in mouse and is also known as H-2Eb, Ia4, or H2Eb. It represents the beta chain of the mouse MHC class II I-A molecule.
• MHC class II molecules are heterodimers composed of an alpha and a beta chain; the beta chain contains the peptide-binding groove and plays a critical role in presenting processed exogenous antigens to CD4+ helper T cells.
• The protein is a member of the immunoglobulin superfamily and is expressed on the surface of professional antigen-presenting cells, including dendritic cells, B cells, and macrophages.
• According to UniProt record P18468, the H2-Eb1 protein is localized to the plasma membrane and contains both a signal peptide and a transmembrane domain, as well as an extracellular immunoglobulin-like domain.
• Key structural features include intrachain disulfide bonds, N-linked glycosylation sites, and potential ubiquitination modifications, which may influence MHC class II antigen presentation and stability.
• Based on associated keywords, the protein is involved in adaptive immune responses, serving as a critical component in the recognition of foreign antigens and the initiation of specific immune reactions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein encompassing the extracellular domain of mouse MHC class II (I-A/I-E), which likely presents native conformational epitopes. For flow cytometry, consider titrating the antibody to determine optimal staining concentrations on relevant antigen-presenting cells such as murine splenocytes or lymph node cells.
• Due to its rat origin, include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding. Assess cross-reactivity with other mouse strains or species as needed; the antibody is reported to react with mouse, but individual allelic variants may exhibit different binding efficiencies.
• The conjugated fluorophore () may be sensitive to light and temperature; protect from light and store at recommended conditions. Validate antibody performance in multicolor flow cytometry panels with appropriate compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This MHC Class II (I-A/I-E) monoclonal antibody supports immunology-focused applications, enabling robust detection of antigen-presenting cells in mouse models. Explore our comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing solutions tailored to your development pipeline.