Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for FC - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The target, H-2 class II histocompatibility antigen I-A beta chain (gene H2-Eb1), is a membrane-localized glycoprotein that forms part of the MHC class II complex.
• It is a key component of adaptive immunity, functioning in the presentation of extracellular antigens to CD4+ T cells.
• The protein is characterized by an N-terminal signal peptide, an extracellular region with a disulfide bond, a transmembrane helix, and a cytoplasmic tail subject to ubiquitination.
• Its three-dimensional structure has been determined, revealing the peptide-binding groove critical for antigen presentation.
• As an MHC class II molecule, it is highly polymorphic, with multiple allelic variants contributing to immune response diversity.

Experimental Guidance and Technical Tips

• The antibody was generated against a recombinant protein, likely preserving native conformation; verify reactivity with your target cell population via appropriate controls.
• Designed for flow cytometry, consider titrating the antibody and optimizing fixation/permeabilization protocols for your assay.
• Cross-reactivity is reported for mouse; for other species, validate experimentally or perform sequence alignment.
• Use isotype-matched control antibodies to assess nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this Rat anti-Mouse MHC Class II (I-A/I-E) monoclonal antibody as part of our comprehensive IVD raw-material portfolio. We support your immunology and diagnostic workflows with one-stop access to validated antibodies, bulk ancillary reagents, and custom sourcing of hard-to-find targets. Rely on CamelBio to streamline your supply chain from concept to clinic.