Rat anti-Mouse Ly6G Monoclonal Antibody for FC - P35461

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G) is a GPI-anchored cell surface glycoprotein, also referred to as Ly-6G.1 or historically as Gr-1 when co-expressed with Ly-6C.
• Encoded by the Ly6g gene, it belongs to the Ly-6/uPAR superfamily and contains a signal peptide required for membrane targeting.
• Ly-6G is predominantly expressed on the cell membrane of specific bone marrow cells, consistent with its role as a myeloid lineage marker.
• The protein exhibits a compact structure stabilized by disulfide bonds and undergoes post-translational modifications including N-glycosylation and GPI-anchor attachment.
• Its GPI-anchor tethers it to lipid rafts on the outer leaflet of the plasma membrane, facilitating its use as a reliable marker for mouse neutrophils.
• Ly-6G is widely utilized in flow cytometry to distinguish granulocytes due to its restricted expression pattern.
• The protein's signal peptide is cleaved during maturation, leaving the mature form anchored extracellularly.
• As a member of the Ly-6 family, it shares structural homology with other GPI-anchored proteins involved in immune cell function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from mouse Ly-6G; verify epitope accessibility in your flow cytometry protocol, as fixation or permeabilization may affect staining.
• offers bright fluorescence suitable for multi-color panels; carefully plan fluorophore combinations to minimize spectral overlap, especially with PE or similar channels.
• While validated for FC, other applications may be feasible after proper validation and optimization in your experimental system.
• Titrate the antibody to determine the optimal concentration for your specific cell type and staining conditions to achieve the best signal-to-noise ratio.
• Because Ly-6G is GPI-anchored, avoid prolonged enzymatic cell dissociation methods that could cleave the anchor and compromise staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For investigations into mouse immunology and neutrophil biology, this anti-Ly6G antibody exemplifies our ability to supply high-quality, application-specific immunological tools. Explore our one-stop sourcing services to streamline your workflow from concept to clinic.