Rat anti-Mouse Ly-6G/Ly-6C Monoclonal Antibody for FC - P35461 / P0CW02

Core Product Specifications and Parameters

Biological Background: Ly-6G/Ly-6C Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also known as Gr-1, is a GPI-anchored cell surface protein belonging to the Ly-6/PLAUR domain-containing protein family.
• The antibody recognizes both Ly-6G (UniProt P35461) and Ly-6C (P0CW02) through a shared epitope within the Gr-1 antigen complex.
• Ly-6G localizes to the cell membrane, anchored by a C-terminal GPI moiety (SUBCELLULAR LOCATION: Cell membrane).
• It is characterized by a signal peptide, disulfide bonds, and N-linked glycosylation, as indicated by UniProt keywords: Cell membrane, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Membrane, Signal.
• Tissue specificity: Expressed in bone marrow, consistent with its expression on developing granulocytes.
• Ly-6C is a closely related GPI-anchored protein expressed on monocytes, dendritic cells, and subsets of T cells, making this antibody valuable for dissecting myeloid and lymphoid populations.

Experimental Guidance and Technical Tips

• The antibody is pre-conjugated with , a bright fluorophore, allowing direct use in flow cytometry (FC) without additional secondary staining steps.
• Titrate the antibody to establish the optimal working dilution for your cell type and experimental conditions; typical starting concentrations range from 0.1–1 µg per 10^6 cells.
• Use appropriate gating strategies to distinguish Ly-6G^hi granulocytes from Ly-6C^hi monocytes in mouse bone marrow, spleen, or peripheral blood samples.
• Due to the recombinant immunogen covering the common Gr-1 epitope, the antibody is expected to bind both Ly-6G and Ly-6C, resulting in broader reactivity than single-target antibodies.
• Include positive controls such as bone marrow granulocytes and negative controls like lymphocyte populations to validate staining specificity.
• As a rat monoclonal antibody, evaluate potential cross-reactivity or background when working with rat-derived samples or in rodent models other than mouse.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering diagnostic manufacturers and labs access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-Ly-6G/Ly-6C monoclonal antibody supports myeloid immunophenotyping in immunology and oncology research; we supply custom conjugations, flexible package sizes, and technical consultation to streamline your assay development from concept to clinical application.