Rat anti-Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC - P35461 / P0CW02

Core Product Specifications and Parameters

Biological Background: Ly-6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also known as Ly-6G.1, is encoded by the Ly6g gene and belongs to the Ly-6 protein family.
• It is a GPI-anchored cell surface glycoprotein, as indicated by its keywords: Glycoprotein, GPI-anchor, Lipoprotein, and Membrane.
• Ly-6G is localized at the cell membrane (SUBCELLULAR LOCATION), consistent with its role as a surface antigen.
• Tissue specificity: It is expressed in bone marrow, where it serves as a marker for granulocyte differentiation.
• The protein contains disulfide bonds, contributing to its structural stability and characteristic Ly-6/uPAR fold.
• The predicted molecular weight of the Ly-6G precursor is approximately 14.3 kDa (mature form ~14 kDa after signal peptide cleavage).
• Ly-6G is commonly recognized together with Ly-6C by the Gr-1 antibody; this product targets an epitope shared between the two antigens.

Experimental Guidance and Technical Tips

• The conjugate enables direct flow cytometry detection; consider titrating the antibody to determine the optimal staining concentration for your cell type.
• Validate staining in mouse bone marrow or peripheral blood samples; Ly-6G is highly expressed on neutrophils and granulocytes, while Ly-6C is present on monocytes, dendritic cells, and some lymphocyte subsets.
• Due to the cross-reactivity with Ly-6C, implement appropriate gating and antibody panels (e.g., co-stain with CD11b, Ly-6C-specific clones) to discriminate granulocytes from monocytic populations.
• As the immunogen was full-length recombinant protein, the recognized epitope may be conformational; ensure that cell preparation and staining conditions preserve native surface proteins.
• Include suitable isotype control antibodies to assess background and non-specific binding in your flow cytometry assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering validated antibodies, antigen pairs, and ancillary reagents that bridge the gap from concept to clinic. This anti-mouse Gr-1 monoclonal antibody is ideal for hematology and immunology research requiring precise granulocyte and monocyte immunophenotyping. For your Ly-6G/Ly-6C assay development, we provide bulk quantities, custom conjugation, and additional reagent solutions to streamline your workflow and accelerate diagnostic innovation.