Rat anti-Mouse F4/80 Monoclonal Antibody for Flow Cytometry - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 Function and Localization

• F4/80, officially designated adhesion G protein-coupled receptor E1 (ADGRE1), is also known as EMR1 hormone receptor, EGF-like module receptor 1, and cell surface glycoprotein F4/80.
• It is an orphan receptor that mediates cell adhesion and likely participates in cell-cell interactions specifically among immune system cells.
• F4/80 has been implicated in the development of regulatory T-cells (Treg).
• The protein localizes to the cell membrane, consistent with its role as a transmembrane receptor.
• Expression is predominantly on macrophages, though it is absent from those within T-cell areas of lymph nodes and spleen; low-level expression is found on blood monocytes.
• Structurally, F4/80 contains multiple EGF-like domains, a GPCR proteolytic site, and is heavily glycosylated, features reflected in keywords including Adaptive immunity, Calcium, Disulfide bond, EGF-like domain, G protein-coupled receptor, Glycoprotein, and Transmembrane helix.
• The mouse gene Adgre1 (GeneID 13733) encodes a 102 kDa precursor protein with a signal peptide, multiple transmembrane regions, and a large extracellular domain.

Experimental Guidance and Technical Tips

• This monoclonal antibody was generated against recombinant mouse F4/80 protein and is validated for flow cytometry (FC).
• When planning FC experiments, titrate the antibody to determine the optimal concentration for your specific sample type and instrument.
• Include appropriate isotype controls and Fc-blocking steps to minimize non-specific binding, especially in macrophage-rich samples.
• For non-FC applications, validate performance in the relevant assay system, as cross-reactivity and sensitivity may differ.
• Store the antibody as recommended (shipped on ice bags) and protect from prolonged light exposure due to the conjugated fluorophore.

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CamelBio supports diagnostic manufacturers and research groups with comprehensive access to IVD raw materials, including monoclonal antibodies like this anti-F4/80 clone, customized antibody pairs, and ancillary reagents for macrophage biology studies. Our platform streamlines procurement of validated bulk reagents and offers tailored sourcing for rare targets, helping you accelerate assay development from concept to clinic.