Rat anti-Mouse F4/80 Monoclonal Antibody for Flow Cytometry - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 (Adgre1) Function and Localization

• F4/80, also known as Adhesion G protein-coupled receptor E1 (Adgre1) or EMR1, is a cell surface glycoprotein belonging to the adhesion GPCR family.
• It functions as an orphan receptor involved in cell adhesion and likely mediates cell-cell interactions within the immune system.
• F4/80 is predominantly expressed on macrophages, but not on those residing in T-cell areas of lymph nodes and spleen; low expression is detected on blood monocytes.
• The protein may contribute to regulatory T-cell (Treg) development.
• It localizes to the cell membrane and features multiple EGF-like domains, a GPCR proteolytic site, and a transmembrane helix.
• Keywords associated with Adgre1 include adaptive immunity, calcium, disulfide bond, glycoprotein, and transducer, underscoring its roles in immune signaling.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein of mouse F4/80; for flow cytometry, consider using macrophage-rich tissues such as spleen or peritoneal lavage for validation.
• This antibody is supplied as an conjugate, enabling direct detection without a secondary antibody. Titrate the antibody to optimize staining for your specific sample type.
• Include appropriate controls (isotype controls, Fc block) to minimize non-specific binding in flow cytometry assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with validated IVD raw materials, including monoclonal antibodies like this anti-F4/80 clone. Our comprehensive supply chain covers validated antibody pairs, optimized conjugates, bulk reagents, and rare target sourcing to accelerate your workflow from concept to clinic. Contact us to explore flexible procurement options tailored to your immunology and macrophage research needs.