Rat anti-Mouse F4/80 Monoclonal Antibody for FC - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 (Adgre1) Function and Localization

• F4/80, encoded by the Adgre1 gene, is also known as EMR1, cell surface glycoprotein F4/80, adhesion G protein-coupled receptor E1, and Ly71.
• It functions as an orphan G protein-coupled receptor involved in cell adhesion and cell-cell interactions, particularly among immune cells.
• F4/80 plays a role in regulatory T-cell (Treg) development, contributing to adaptive immunity.
• The protein is predominantly expressed on macrophages, but is absent from macrophages within T-cell areas of lymph nodes and spleen; low-level expression is observed on blood monocytes.
• F4/80 is localized to the cell membrane and possesses structural features including EGF-like domains, a GPCR proteolytic site, and multiple transmembrane helices.
• Post-translational modifications include glycosylation and disulfide bond formation, as indicated by keywords.
• The molecular weight is approximately 102 kDa.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated with , making it suitable for flow cytometry without a secondary antibody. Ensure that the fluorophore is compatible with your instrument’s laser and filter configuration.
• Since the immunogen is a recombinant protein representing full-length mouse F4/80, the antibody may recognize linear or conformational epitopes; performance may vary in paraformaldehyde-fixed samples—consider testing under different fixation/permeabilization conditions.
• As F4/80 is a cell surface marker, live-cell staining is recommended for optimal signal; titrate the antibody to determine the optimal concentration for your specific cell type and panel design.
• Cross-reactivity is reported for mouse; for other species, validate in the relevant sample system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, connecting diagnostic manufacturers and research laboratories with validated antibodies, bulk reagents, and rare-target resources. For macrophage biology and immune cell targeting, we supply high-quality anti-F4/80 monoclonal antibodies, along with complementary reagents and technical support to streamline your assay development from concept to clinic.