Rat anti-Mouse CD45R/B220 Monoclonal Antibody for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45R/B220 (PTPRC) Function and Localization

• CD45R/B220 is the mouse homolog of the receptor-type tyrosine-protein phosphatase C (PTPRC), also known as leukocyte common antigen (L-CA) and Ly-5.
• It is a transmembrane protein-tyrosine phosphatase essential for T-cell activation through the antigen receptor, acting as a positive regulator of coactivation upon binding to DPP4.
• The protein contains two PTPase domains: the first possesses enzymatic activity, while the second modulates substrate specificity.
• Upon T-cell activation, PTPRC recruits and dephosphorylates SKAP1 and FYN, and also dephosphorylates LYN to modulate its activity.
• It localizes to the cell membrane, membrane rafts, and immunological synapses, underscoring its role in signal transduction.
• Multiple isoforms are produced by alternative splicing, contributing to functional diversity in different leukocyte subsets.
• The specific epitope recognized by this antibody (CD45R/B220) is a B-cell isoform of PTPRC, making it a valuable marker for B lymphocyte studies.
• Additional post-translational modifications include glycosylation and phosphorylation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal dilution for your specific cell type and experimental conditions.
• Include appropriate isotype controls (rat IgG) and perform Fc receptor blocking to minimize non-specific binding.
• CD45R/B220 is expressed on B cells from pro-B to mature stages but is absent on plasma cells; verify expression in your sample population.
• As the antibody is raised against recombinant protein, consider confirming specificity using PTPRC knockout or knockdown models if available.
• When designing multicolor panels, check fluorophore compatibility and assess potential spectral overlap with other markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering a comprehensive portfolio that includes validated monoclonal antibodies such as this anti-CD45R/B220 clone for immunology and B-cell research. Beyond single antibodies, we support diagnostic manufacturers with optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare leukocyte targets—helping you advance from concept to clinic seamlessly.