Rat anti-Mouse CD45R/B220 Monoclonal Antibody for FC - P06800

Core Product Specifications and Parameters

Biological Background: CD45R/B220 Function and Localization

• CD45R/B220, encoded by the Ptprc gene, is the mouse homolog of human CD45, also known as leukocyte common antigen (L-CA) or Ly-5. It exists in multiple isoforms generated by alternative splicing, with molecular weights of approximately 130, 140, and 144 kDa.
• Functions as a receptor-type tyrosine-protein phosphatase, essential for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4.
• The protein contains two phosphatase domains: the first has enzymatic activity, while the second modulates substrate specificity. Upon T-cell activation, it recruits and dephosphorylates signaling molecules such as LYN, SKAP1, and FYN.
• CD45R/B220 is localized to the cell membrane, membrane rafts, and immunological synapse, consistent with its role in modulating immune cell signaling at sites of cell-cell contact.
• Post-translational modifications include extensive glycosylation and phosphorylation, and the protein undergoes alternative splicing, generating distinct isoforms with regulated expression during lymphocyte development.
• Keywords: Alternative splicing, Glycoprotein, Hydrolase, Membrane, Phosphoprotein, Protein phosphatase, Synapse, Transmembrane.

Experimental Guidance and Technical Tips

• Consider titrating the antibody to determine the optimal concentration for flow cytometry staining of mouse hematopoietic cells.
• Validate antibody specificity using appropriate negative controls, such as cells from Ptprc knockout mice or isotype control antibodies.
• Be aware that CD45R/B220 exhibits multiple isoforms; confirm that the antibody recognizes the isoform relevant to your experimental context.
• As a rat-derived antibody, ensure compatibility of anti-rat secondary antibodies if required for signal amplification in indirect flow cytometry protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD45R/B220 antibody is part of our expanding portfolio for immuno-oncology and hematology research. Beyond validated antibody pairs, we offer optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.