Rat anti-Mouse CD45R/B220 mAb for Flow Cytometry - P06800

Core Product Specifications and Parameters

Biological Background: CD45R/B220 (Receptor-type tyrosine-protein phosphatase C) Function and Localization

• CD45R/B220, encoded by the Ptprc gene, is a receptor-type tyrosine-protein phosphatase C (PTPRC), also known as leukocyte common antigen (L-CA), lymphocyte antigen 5 (Ly-5), and T200. Multiple isoforms are generated through alternative splicing, producing variants of different molecular weights (e.g., 130, 140, 144 kDa).
• PTPRC functions as a critical positive regulator of T-cell activation: its first phosphatase domain has enzymatic activity, while the second one modulates substrate specificity. Upon T-cell receptor engagement, it recruits and dephosphorylates signaling molecules such as SKAP1 and FYN (by similarity).
• It also dephosphorylates the Src-family kinase LYN, thereby modulating LYN kinase activity and influencing downstream signaling cascades in lymphocytes.
• Subcellularly, PTPRC is localized to the cell membrane, enriched in membrane lipid rafts, and concentrates at the immunological synapse between T cells and antigen-presenting cells.
• PTPRC interacts with CLEC10A on immature dendritic cells at the T cell–dendritic cell interface; this interaction recognizes Tn antigen-carrying CD45 on effector T cells, adjusting the threshold for T cell activation to limit autoreactivity.
• The protein undergoes glycosylation and phosphorylation, as indicated by the keywords Glycoprotein and Phosphoprotein. It is a type I transmembrane protein with a signal peptide, a large extracellular domain, a single transmembrane helix, and two intracellular phosphatase domains.
• In mice, CD45R/B220 is predominantly expressed on B lymphocytes and a subset of T cells, making it a classic marker for B cell lineages in immunological studies.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the -conjugated antibody to determine the optimal concentration for your specific cell type and staining protocol, as non‑specific binding can be reduced by proper titration.
• Include appropriate isotype controls (rat IgG conjugated to the same fluorophore) to set compensation and gating parameters in multicolor panels.
• Validate antibody performance on known positive and negative cell populations (e.g., mouse splenocytes where B cells are CD45R/B220+ and T cell subsets are mostly negative).
• Due to the large extracellular domain and multiple isoforms, ensure that the recombinant immunogen corresponds to the epitope recognized by this clone if cross‑reactivity across isoforms is critical.
• Store the antibody at 2–8°C protected from light; avoid repeated freeze‑thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials. This anti‑mouse CD45R/B220 monoclonal antibody supports immunophenotyping and cell analysis applications, and CamelBio can fulfill bulk orders with consistent lot‑to‑lot performance. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, ancillary reagents, and rare‑target sourcing services to cover your entire development pipeline from concept to clinic.