Rat anti-Mouse CD3 Monoclonal Antibody for Flow Cytometry - P04235 / P22646

Core Product Specifications and Parameters

Biological Background: CD3 (delta and epsilon chains) Function and Localization

• CD3 delta (gene Cd3d) and epsilon (Cd3e) are essential components of the T-cell receptor (TCR)-CD3 complex on the surface of T lymphocytes, playing a central role in adaptive immunity.
• The CD3 complex (delta, epsilon, gamma, and CD247/zeta chains) transduces activation signals upon TCR engagement by antigen-presenting cells, via phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains.
• CD3D is critical for thymocyte differentiation and proper surface expression of the TCR-CD3 complex; disruption impairs T-cell development. Related references: PMID:10935641
• CD3 delta interacts with coreceptors CD4 and CD8, functionally linking TCR recognition to co-receptor signaling, which is required for activation and positive selection of CD4+ or CD8+ T cells.
• Both CD3 delta and epsilon chains are type I transmembrane glycoproteins localized to the plasma membrane.
• Key post-translational modifications include disulfide bond formation, glycosylation, and ITAM phosphorylation by Src family kinases LCK and FYN.
• Together, CD3 delta and epsilon chains have molecular weights of approximately 19 kDa and 21 kDa, respectively.

Experimental Guidance and Technical Tips

• The fluorescent conjugate enables direct detection in flow cytometry (FC) without the need for secondary staining reagents.
• To reduce non-specific binding, consider pre-incubating cells with an appropriate Fc receptor blocking reagent and use a matched rat IgG isotype control.
• Optimize antibody concentration by titration over a range (e.g., 0.1–5 µg/mL) in the relevant cell system to achieve the best signal-to-noise ratio.
• Prepare single-cell suspensions and assess viability before staining to ensure robust and reproducible results.
• The antibody is recommended for use with mouse samples; validate assay conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for T-cell immunology research, offering this anti-mouse CD3 antibody alongside a comprehensive portfolio of validated reagents. From optimized monoclonal/polyclonal antibodies and bulk ancillary materials to rare target sourcing, we provide end-to-end support to accelerate your diagnostic development from concept to clinic.