RASGRP3 Rabbit pAb - Q8IV61

Core Product Specifications and Parameters

Biological Background: RASGRP3 Function and Localization

• RASGRP3 is a member of the Ras guanyl-releasing protein family that functions as a guanine nucleotide exchange factor (GEF) for Ras and Rap1.
• It catalyzes the exchange of GDP for GTP, thereby activating Ras and Rap1 signaling pathways.
• The protein is regulated by calcium and diacylglycerol (DAG), as indicated by its alternative name "Calcium and DAG-regulated guanine nucleotide exchange factor III".
• RASGRP3 contains zinc-finger motifs and calcium-binding domains, suggesting metal-dependent regulation.
• Alternative splicing results in multiple transcript variants (Keywords: Alternative splicing), potentially giving rise to different isoforms.
• Annotated keywords include "Guanine-nucleotide releasing factor", "Zinc", "Zinc-finger", and "Calcium".

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 540-689); consider verifying isoform reactivity if alternative splicing affects this domain.
• For WB, a band near the predicted 78 kDa (UniProt: 78332 Da) may be detected. Observed molecular weight can shift due to post-translational modifications.
• In IF/ICC, optimize fixation and permeabilization conditions to ensure proper RASGRP3 detection.
• For ELISA, this polyclonal antibody can serve as capture or detection reagent; pairing with a monoclonal antibody may enhance specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research labs with a one-stop supply of IVD raw materials. This anti-RASGRP3 rabbit polyclonal antibody supports Ras/Rap1 signaling research and assay development. From validated antibody pairs to bulk ancillary reagents, CamelBio streamlines sourcing for projects from concept to clinic.