Raptor Rabbit Polyclonal Antibody for WB, ELISA - Q8N122

Core Product Specifications and Parameters

Biological Background: Raptor Function and Localization

• Raptor (Regulatory-associated protein of mTOR, also known as p150 TOR-scaffold protein) is a key component of the mTORC1 complex, serving as both a molecular adapter for lysosomal recruitment and a substrate-specific adapter that directs TOS motif-containing proteins to the MTOR kinase domain for phosphorylation. Related references: PMID:12747827, PMID:24403073, PMID:26588989
• mTORC1 is an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis, promoting protein, lipid, and nucleotide synthesis via phosphorylation of substrates like RPS6KB1 and EIF4EBP1. Related references: PMID:12150925, PMID:12150926, PMID:12747827
• mTORC1 also inhibits catabolic pathways by phosphorylating autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulator of lysosomal biogenesis and autophagy. Related references: PMID:12150925, PMID:12150926, PMID:12747827
• The RPTOR gene encodes multiple isoforms via alternative splicing, and the protein contains WD repeats and undergoes post-translational modifications including acetylation, phosphorylation, glycosylation, and ubiquitination.
• Raptor is localized primarily to the lysosome membrane, cytoplasm, and cytoplasmic granules, consistent with its role in mTORC1 lysosomal recruitment and signaling.
• Raptor is widely expressed, with highest levels in skeletal muscle, nasal mucosa, and pituitary, and lower levels in spleen and other tissues, reflecting its role in growth and metabolism.
• mTORC1 regulates diverse processes such as odontoblast and osteoclast differentiation, neuronal transmission, and prosocial behavior induced by LSD, highlighting its broad physiological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region in the C-terminal part of human Raptor (aa 888-1013), suggesting the antibody may recognize full-length protein and possibly cleaved fragments containing this region.
• For Western blot, a band around 149 kDa is expected; consider using appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• For ELISA, the antibody can be used as capture or detection reagent; pairing with another clone may be needed for sandwich assays.
• Validate cross-reactivity in mouse and rat samples, as reactivity is predicted based on sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For mTOR pathway research and anti-Raptor antibody sourcing, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with CamelBio to streamline your IVD development with reliable and scalable biological resources.