Raptor Rabbit Polyclonal Antibody for WB, ELISA - Q8N122

Core Product Specifications and Parameters

Biological Background: Raptor Function and Localization

• Raptor (RPTOR), also known as p150 target of rapamycin (TOR)-scaffold protein, is a core component of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:12150925, PubMed:12150926, PubMed:12747827).
  Related references: PMID:12150925, PMID:12150926, PMID:12747827
• Within mTORC1, Raptor acts as a molecular adapter that mediates recruitment of the complex to lysosomal membranes via interaction with small GTPases (RagA/B/C/D) and promotes substrate specificity by binding to TOS motif-containing proteins, directing them toward the MTOR kinase domain for phosphorylation (PubMed:12747827, PubMed:24403073, PubMed:26588989).
  Related references: PMID:12747827, PMID:24403073, PMID:26588989
• In response to nutrients, growth factors, or amino acids, mTORC1 phosphorylates downstream substrates such as ribosomal protein S6 kinases (RPS6KB1, RPS6KB2) and EIF4EBP1 (4E-BP1) to promote protein, lipid, and nucleotide synthesis, while inhibiting catabolic pathways by phosphorylating autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB (PubMed:12150925, PubMed:12150926, PubMed:12747827).
  Related references: PMID:12150925, PMID:12150926, PMID:12747827
• Raptor is primarily localized to the lysosome membrane, cytoplasm, and cytoplasmic granules, consistent with its role in nutrient sensing and signaling.
• The protein is highly expressed in skeletal muscle, with moderate levels in brain, lung, small intestine, kidney, and placenta.
• Raptor undergoes multiple post-translational modifications, including phosphorylation, acetylation, glycation, ISGylation (Ubl conjugation), and isopeptide bond formation, regulating its activity and interactions (Keywords: Acetylation, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation).
• It is also associated with host-virus interactions and exists in alternatively spliced isoforms, expanding its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 888-1013 of human Raptor, a region that may be conserved across species; validate cross-reactivity in your models.
• For Western blot (WB), a band near 149 kDa is expected; optimize transfer conditions for large proteins.
• For ELISA, consider using the antibody in a capture or detection format; optimal dilutions should be determined experimentally.
• As a polyclonal antibody, it may offer robust signal amplification; however, validate specificity in knockout or knockdown controls.
• The antibody reacts with human, mouse, and rat Raptor; if using other species, check sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to premium IVD raw materials, including validated antibodies for mTOR signaling and autophagy pathways. Our portfolio includes optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities, streamlining your development from concept to clinic. Contact us to secure reliable supply chains for your Raptor-related research and assay needs.