RAPGEF6 Rabbit Polyclonal Antibody for WB, ELISA - Q8TEU7

Core Product Specifications and Parameters

Biological Background: RAPGEF6 Function and Localization

• RAPGEF6, also known as PDZ-GEF2, RA-GEF-2, and PDZGEF2, is a ~179 kDa multidomain protein that functions as a guanine nucleotide exchange factor (GEF).
• It specifically activates Rap1A, Rap2A, and M-Ras GTPases by catalyzing the exchange of GDP for GTP, but does not interact with cAMP.
• The protein localizes primarily to the cytoplasm and associates with the cell membrane, consistent with its role in signal transduction at the plasma membrane.
• Alternative splicing generates multiple isoforms; isoform 3 is predominantly expressed in brain, heart, liver, lung, and placenta, with minimal detection in skeletal muscle, kidney, and pancreas.
• RAPGEF6 undergoes post-translational modifications including phosphorylation and acetylation, as identified through proteomic studies (Keywords: Acetylation, Phosphoprotein).
• A 3D structure has been determined, revealing critical domains for nucleotide exchange activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 145-240 of human RAPGEF6 (NP_001157858), which may target a conserved epitope; cross-reactivity with mouse and rat is confirmed.
• For WB, consider using lysates from tissues with high RAPGEF6 expression such as brain or heart, and optimize antibody dilution based on the recombinant immunogen specificity.
• ELISA experiments may benefit from using the recombinant protein fragment as a positive control to assess binding efficiency.
• As this is a polyclonal antibody, batch-to-batch consistency should be verified in the intended assay system.

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CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this RAPGEF6 antibody, and bulk ancillary reagents to support research into GTPase signaling pathways. With a broad supply network covering rare target reagents and custom sourcing, CamelBio helps diagnostic manufacturers and labs accelerate development from concept to clinic.