RanGAP1 Rabbit pAb - P46060

Core Product Specifications and Parameters

Biological Background: RanGAP1 Function and Localization

• Encoded by the RANGAP1 gene (synonyms: SD, KIAA1835); a 587‑amino acid human protein with a predicted mass of ~64 kDa (UniProt: 63.5 kDa).
• Functions as a GTPase‑activating protein (GAP) for Ran, converting cytoplasmic Ran‑GTP to Ran‑GDP, a step essential for Ran‑dependent nuclear import and export. Related references: PMID:16428860, PMID:8146159, PMID:8896452, PMID:27160050
• Mediates the dissociation of cargo from nuclear export complexes containing XPO1, Ran, and RANBP2 after nuclear export. Related references: PMID:27160050
• Contains leucine‑rich repeats and undergoes multiple post‑translational modifications including acetylation, phosphorylation, and ubiquitin‑like conjugation (e.g., SUMOylation), which regulate its localization and interactions.
• Localizes to the cytoplasm, nucleoplasm, nuclear envelope, centromeres/kinetochores, and the mitotic spindle.
• Highly expressed in the brain, thymus, and testis.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 398‑587) shares high homology between human and mouse, supporting the observed cross‑reactivity; verify reactivity in your specific model system.
• For Western blot, expect a band near 64 kDa; include appropriate positive controls such as HeLa, Jurkat, or mouse brain lysates.
• For ELISA, consider validating against the full‑length protein or the recombinant fragment in the relevant sample matrix.
• To confirm specificity, siRNA knockdown or knockout validation is recommended for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and IVD developers with streamlined access to raw materials, including validated antibody pairs and optimized polyclonal antibodies such as this RanGAP1 rabbit pAb. Whether you need bulk ancillary reagents or assistance sourcing rare‑target materials for nuclear transport or cell cycle studies, CamelBio bridges your project from concept to clinic.