RanGAP1 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P46060

Core Product Specifications and Parameters

Biological Background: RanGAP1 Function and Localization

• RanGAP1 (Ran GTPase-activating protein 1) serves as a GTPase activator for the small GTPase RAN, accelerating the conversion of GTP-bound RAN to GDP-bound RAN in the cytoplasm, a critical step for Ran-dependent nuclear transport. Related references: PMID:16428860 PMID:8146159 PMID:8896452 PMID:27160050
• It mediates the dissociation of export cargo from nuclear export complexes that contain XPO1, RAN, and RANBP2 following nuclear export. Related references: PMID:27160050
• The protein localizes to multiple cellular compartments, including the cytoplasm, nucleoplasm, nuclear envelope, centromeres, kinetochores, and the mitotic spindle, reflecting its role in both interphase transport and mitosis.
• Highly expressed in brain, thymus, and testis, indicating tissue-specific regulation of RanGAP1 activity.
• RanGAP1 contains leucine-rich repeats and undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination, which may modulate its function and stability.
• The human RanGAP1 protein is 587 amino acids in length with a predicted molecular weight of ~63.5 kDa (observed at approximately 64 kDa by SDS-PAGE).

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 398–587, a portion of the C-terminal region of RanGAP1; therefore, the antibody may recognize full-length RanGAP1 as well as any cleavage fragments containing this epitope.
• For Western blot analysis, a predominant band near 64 kDa is expected in human cell lysates or tissue extracts; consider including positive controls such as HeLa, HEK293, or testis lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody in human tissues or cell lines with known RanGAP1 expression (e.g., testis, brain); optimization of antigen retrieval and permeabilization conditions is recommended.
• For ELISA applications, use recombinant RanGAP1 protein or a peptide derived from the immunogen sequence as a coating antigen to assess antibody specificity and titer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering one-stop access to validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-RanGAP1 polyclonal antibody supports research into nucleocytoplasmic transport and cell division, and our team can assist with technical consulting, scale-up supply, and integrated solutions from discovery to clinical application. Leverage CamelBio's expertise to accelerate your diagnostic development and research programs.