RalA Rabbit Monoclonal Antibody for WB, ELISA - P11233

Core Product Specifications and Parameters

Biological Background: RalA Function and Localization

• RalA is a multifunctional small GTPase that interacts with distinct downstream effectors to regulate gene expression, cell migration, proliferation, oncogenic transformation, and membrane trafficking. Related references: PMID:18756269 PMID:19306925 PMID:20005108
• Serves as a GTP sensor for GTP-dependent exocytosis of dense core vesicles and, via the RALA-exocyst complex, controls integrin-dependent membrane raft exocytosis and growth signaling. Related references: PMID:20005108
• Functions as a key regulator of LPAR1 signaling by competing with GRK2 for LPAR1 binding, thereby modulating receptor signaling properties.
• Required for anchorage-independent proliferation of transformed cells. Related references: PMID:19306925
• During mitosis, localizes to the cleavage furrow and midbody, where it supports stabilization and elongation of the intracellular bridge and recruits exocyst components via EXOC2. Related references: PMID:18756269
• Also at the midbody, controls mitochondrial fission by recruiting RALBP1, leading to phosphorylation and activation of DNM1L by cyclin B-CDK1. Related references: PMID:21822277
• Subcellular localization includes the cell membrane, cleavage furrow, midbody, midbody ring, and mitochondrion.
• Post-translational modifications include glycosylation, prenylation, methylation, and phosphorylation; belongs to the small GTPase superfamily with a nucleotide-binding domain.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein covering amino acids 120–206 of human RalA, targeting the C-terminal region. This design may yield antibodies that recognize full-length RalA and possibly isoforms or splice variants containing this sequence.
• In Western blot, a band at approximately 24 kDa is expected for endogenous RalA. We recommend optimizing lysis conditions and using appropriate controls (e.g., RalA-overexpressing lysates) to confirm specificity, especially given possible co-migration with other small GTPases.
• The antibody is reactive to human and monkey RalA; cross-reactivity with other species should be experimentally verified if needed.
• For ELISA, the antibody can be employed as a capture or detection reagent; titrate and validate with recombinant RalA protein to establish assay sensitivity and linear range.
• As a monoclonal rabbit antibody, it offers high specificity and lot-to-lot consistency, suitable for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop supplier of IVD raw materials, supporting diagnostic manufacturers with high-quality antibodies and ancillary reagents. For researchers focusing on GTPase signaling and oncogenic pathways, CamelBio can source extensively validated RalA antibody clones, matched pairs, and recombinant proteins to streamline assay development. Whether you need bulk monoclonal antibodies or rare target reagents, CamelBio bridges your concept to clinic.