RAD54L Rabbit Polyclonal Antibody for WB and ELISA - Q92698

Core Product Specifications and Parameters

Biological Background: RAD54L Function and Localization

• RAD54L (also known as hHR54, hRAD54, RAD54A) is a multifunctional ATPase that plays a central role in homologous recombination (HR), a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA gaps, and stalled or collapsed replication forks.
  Related references: PMID:11459989, PMID:12205100, PMID:24798879
• It acts as a molecular motor, converting the passive diffusion-based homology search into an active, motor-guided process by translocating RAD51-coated ssDNA along donor dsDNA.
• Essential for the formation of RAD51-mediated synaptic complexes, a three-stranded nucleoprotein filament that stabilizes homologous pairing once sequence identity is recognized.
• After strand exchange, RAD54L promotes the dissociation of RAD51 from dsDNA, facilitating the completion of the recombination cycle.
• Localized predominantly in the nucleus, consistent with its DNA repair function.
• Belongs to the SWI/SNF2 family of helicase-like ATPases and contains a zinc-finger DNA-binding domain. Post-translationally modified by acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 648–747) of human RAD54L. For Western blot, expect a band at ~84 kDa; verify with a positive control such as HeLa or HEK293 nuclear extract.
• Cross-reactivity with mouse and rat enables its use in rodent model studies; however, always validate species-specific detection conditions.
• For WB, start with a 1:1000 dilution and optimize as needed; for ELISA, test titers to determine optimal coating or detection concentration.
• Ensure thorough nuclear protein extraction, as RAD54L is a nuclear protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories by providing one-stop access to high-quality IVD raw materials. This RAD54L antibody supports DNA repair and genomic stability studies, and our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities to accelerate your assay development from concept to clinic.