Rad52 Rabbit mAb for WB, ELISA - P43351

Core Product Specifications and Parameters

Biological Background: RAD52 Function and Localization

• RAD52 is a DNA repair protein that plays a central role in homologous recombination and the repair of DNA double-strand breaks.
• It promotes the annealing of complementary single-stranded DNA and stimulates the activity of the RAD51 recombinase, facilitating strand exchange.
• RAD52 is predominantly localized in the nucleus, where it exerts its DNA repair functions.
• The protein undergoes alternative splicing, resulting in multiple isoforms with molecular weights of approximately 13, 15, 25, and 46 kDa.
• RAD52 is a DNA-binding phosphoprotein, and its phosphorylation status may regulate its activity in response to DNA damage.
• Key biological processes associated with RAD52 include DNA damage response, DNA recombination, and DNA repair, underscoring its importance in maintaining genomic integrity.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a synthetic peptide corresponding to the C-terminal region (amino acids 319-418) of human RAD52. When performing Western blot (WB), consider using nuclear extracts from human cell lines as a positive control to verify the presence of RAD52 isoforms.
• Due to alternative splicing, multiple bands may be observed. Validate the specificity of the antibody in your experimental system by comparing with known molecular weight markers or knockout/knockdown samples.
• For ELISA applications, the peptide immunogen can be employed as a coating antigen to assess antibody reactivity. Optimize coating concentrations and blocking conditions for best results.
• Cross-reactivity is reported for human; reactivity with other species has not been determined. If using samples from other species, validate the antibody’s performance empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to validated IVD raw materials, including high-specificity monoclonal antibodies like this anti-RAD52 clone. Our procurement network enables reliable sourcing of DNA repair–focused antibody pairs, bulk ancillary reagents, and custom rare-target development to support your assay needs from research through clinical application. Partner with us to bridge your product development gap efficiently.