Rad51D Rabbit mAb for WB and ELISA - O75771

Core Product Specifications and Parameters

Biological Background: RAD51D Function and Localization

• DNA repair protein RAD51 homolog 4 (RAD51D), also known as RAD51 homolog D, RAD51-like protein 3 (RAD51L3), TRAD, R51H3, and BROVCA4, is encoded by the RAD51D gene (GeneID: 5892).
• Plays a critical role in homologous recombination repair (HRR) of double-stranded DNA breaks, binding single-stranded DNA and exhibiting DNA-dependent ATPase activity.
• Component of the RAD51 paralog protein complex BCDX2 (RAD51B-RAD51C-RAD51D-XRCC2), which operates in the BRCA1-BRCA2-dependent HR pathway, acting downstream of BRCA2 loading and upstream of RAD51 filament formation.
• BCDX2 complex binds to Holliday junctions and replication fork junctions, and the RAD51D-XRCC2 subcomplex can stimulate Holliday junction resolution by BLM helicase.
• Involved in telomere maintenance.
• Localizes to the nucleus, cytoplasm, centrosome, and telomeric regions of chromosomes.
• Expressed in a variety of tissues including colon, prostate, spleen, testis, ovary, thymus, and small intestine, with weak expression in leukocytes.
• Classified under keywords: Alternative splicing, ATP-binding, DNA damage, DNA repair, DNA recombination, DNA-binding, Nucleotide-binding, and Telomere.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1‑120) of human RAD51D (O75771), which may enable recognition of full‑length RAD51D and possibly isoforms sharing this domain. Consider sequence alignment to assess cross‑reactivity with paralogs such as RAD51, RAD51B, and RAD51C if needed.
• For Western blotting, RAD51D has a predicted molecular weight of approximately 35 kDa; ensure sufficient separation and transfer conditions for proteins in this size range.
• For ELISA applications, the recombinant immunogen can serve as a positive control, and paired with appropriate secondary antibodies, the antibody can be used for quantitative or qualitative detection of RAD51D.
• Given the cross‑reactivity with human, mouse, and rat samples, validate antibody specificity and sensitivity in the relevant tissue or cell line before formal experiments.

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