RABIF Rabbit pAb for WB, IF/ICC, ELISA - P47224

Core Product Specifications and Parameters

Biological Background: RABIF Function and Localization

• RABIF (also known as MSS4, RASGFR3, RASGRF3) is a guanine nucleotide exchange factor (GEF) that specifically acts on members of the SEC4/YPT1/RAB subfamily, promoting GDP/GTP exchange and activation of Rab GTPases.
• Stimulates GDP release from YPT1 (Rab1), RAB3A, and RAB10, though its activity is less potent on these targets compared to SEC4; this GEF activity implicates RABIF in the regulation of diverse vesicular transport pathways (PubMed:31540829). Related references: PMID:31540829
• Might play a general role in intracellular vesicular transport due to its broad Rab substrate specificity.
• Ubiquitously expressed across tissues, suggesting a fundamental cellular function in membrane trafficking.
• Contains a zinc-binding domain, indicative of structural metal coordination important for protein stability or function.
• Subject to acetylation, a post-translational modification that may regulate its activity or interactions.
• The protein has a known 3D structure, providing insights into its GEF mechanism and substrate recognition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RABIF (aa 1-123), which is highly conserved; cross-reactivity with mouse and rat suggests recognition of conserved epitopes. Validate specificity in the relevant species and sample type.
• As a polyclonal antibody, it may detect multiple isoforms or related family members; for applications requiring high specificity, consider using blocking peptides or siRNA knockdown controls.
• For WB, a band around 14 kDa is expected; optimization of antibody dilution and blocking conditions is recommended for best signal-to-noise ratio.
• This antibody is suitable for IF/ICC; ensure proper fixation and permeabilization protocols, as localization signals may be impacted by overexpression of tagged proteins.
• In ELISA, use as capture or detection antibody; proper titration and cross-reactivity testing against closely related GEFs is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This anti-RABIF polyclonal antibody is part of our extensive portfolio for vesicular transport and GTPase signaling research. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio is your trusted partner in accelerating diagnostic development.