Rabbit anti-ZNF217 Polyclonal Antibody for WB, IF/ICC, ELISA - O75362

Core Product Specifications and Parameters

Biological Background: ZNF217 Function and Localization

• Protein Identity: Zinc finger protein 217 (ZNF217), also known as ZABC1, is a member of the zinc finger protein family. It contains multiple C2H2-type zinc finger domains that mediate DNA binding and transcriptional regulation.
• Transcriptional Repressor: ZNF217 binds to the promoters of target genes and functions as a transcriptional repressor, modulating gene expression to promote cell proliferation and inhibit apoptosis.
• Cell Proliferation and Survival: The protein plays a role in promoting cell proliferation and antagonizing cell death. It enhances phosphorylation of AKT1 at Ser-473, activating the PI3K/AKT signaling pathway.
• Subcellular Localization: ZNF217 is localized in the nucleus, consistent with its role as a transcription factor.
• Post-Translational Modifications: According to UniProt keywords, ZNF217 is subject to phosphorylation and isopeptide bond formation (ubiquitination), suggesting regulation by these modifications.
• Structural Features: The protein has a predicted molecular weight of approximately 115 kDa and belongs to the Krueppel C2H2-type zinc-finger protein family. It contains multiple zinc finger repeats that stabilize its DNA-binding structure.

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was raised against a recombinant protein covering amino acids 1-190 of human ZNF217 (N-terminal region). This region may contain epitopes that are exposed and conserved across species, explaining the observed cross-reactivity with mouse and rat.
• Western Blotting (WB): For WB, separate proteins on a suitable percentage SDS-PAGE gel (e.g., 8–10%) to resolve the ~115 kDa ZNF217 band. Validate primary antibody dilution empirically; typical ranges for polyclonal antibodies are 1:500–1:2000.
• Immunofluorescence/Immunocytochemistry (IF/ICC): Since ZNF217 is nuclear, use a permeabilization step (e.g., 0.1% Triton X-100) during fixation to allow antibody access. Include a nuclear counterstain (e.g., DAPI) to confirm localization.
• ELISA: The recombinant immunogen can serve as a positive control in ELISA. Optimize coating concentration and antibody dilution based on preliminary checkerboard titrations.
• Cross-Reactivity: Cross-reactivity with mouse and rat ZNF217 is reported. Verify by sequence alignment of the N-terminal region to assess epitope conservation. Validate in relevant cell lines or tissues from these species.

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