Rabbit anti-ZIC1 Polyclonal Antibody for WB, ELISA - Q15915

Core Product Specifications and Parameters

Biological Background: ZIC1 Function and Localization

• ZIC1 is a zinc finger transcriptional activator that plays essential roles in neurogenesis and the early development of the central nervous system (CNS), including dorsal spinal cord specification and cerebellum maturation.
• It regulates mossy fiber neuron migration and axon guidance within the pontine gray nucleus, contributing to the spatial organization of cerebellar circuitry.
• ZIC1 may participate in shear flow mechanotransduction in osteocytes, suggesting potential roles outside the nervous system.
• It retains the transcriptional effectors GLI1 and GLI3 in the cytoplasm and directly binds the minimal GLI-consensus DNA sequence 5'-TGGGTGGTC-3', modulating hedgehog pathway outputs.
• The protein primarily localizes to the nucleus, with additional cytoplasmic distribution.
• Tissue expression is predominantly in the CNS, with high levels in the cerebellum; it is detected in cerebellar granule cell lineages and in the majority of medulloblastoma cases.
• ZIC1 is classified under multiple keywords including activator, developmental protein, DNA-binding, zinc-finger, and disease variant (associated with craniosynostosis and intellectual disability).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 120–210 of human ZIC1; consider aligning this region with orthologs or isoforms to assess potential cross-reactivity.
• For Western blot, a band around 48 kDa is expected; validate detection in samples known to express ZIC1, such as cerebellar or neuronal cell lysates.
• For ELISA, optimize coating and detection conditions, and include appropriate controls to ensure specificity.
• All applications should be verified in the relevant sample system and experimental setup to confirm performance under your specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets like ZIC1. With a focus on neurodevelopmental and cancer research markers, we support diagnostic manufacturers and laboratories at every stage—from concept to clinic—ensuring reliable access to critical raw materials and technical consultation.