Rabbit Anti-THUMPD3 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BV44

Core Product Specifications and Parameters

Biological Background: THUMPD3 Function and Localization

• THUMPD3 is a THUMP domain-containing protein and the catalytic subunit of the THUMPD3-TRM112 methyltransferase complex.
• It specifically mediates S-adenosyl-L-methionine-dependent N2-methylation of guanosine at position 6 (m2G6) in tRNAs, and also catalyzes m2G7 methylation in tRNA(Trp). Related references: PMID:34669960 PMID:37283053
• Subcellular localization is predominantly cytoplasmic, consistent with its role in tRNA maturation.
• Belongs to the class I-like SAM-binding methyltransferase superfamily, featuring an RNA-binding THUMP domain.
• Keywords: Methyltransferase, RNA-binding, tRNA processing, S-adenosyl-L-methionine, Transferase, Cytoplasm.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to residues 1-250 of human THUMPD3, a region covering the N-terminal part of the protein. For applications requiring epitope specificity, consider mapping or competitive assays.
• For Western blot (WB), THUMPD3 has a predicted molecular weight of approximately 57 kDa. Use appropriate loading controls and validate with lysates from human, mouse, or rat samples.
• In immunohistochemistry-paraffin (IHC-P), optimize antigen retrieval and antibody dilution; include isotype controls and tissue sections known to express THUMPD3.
• When using this antibody in ELISA, consider pairing with a matched detection antibody and validate with recombinant protein or peptide standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including this THUMPD3 antibody, supporting your tRNA modification research. Our portfolio encompasses validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing assistance, enabling smooth transitions from concept to clinical application.