Rabbit Anti-SPDYA Polyclonal Antibody for WB and ELISA - Q5MJ70

Core Product Specifications and Parameters

Biological Background: SPDYA Function and Localization

• Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2. Related references: PMID:12972555
• Contributes to CDK2 activation without promoting CDK2 phosphorylation by inducing a conformational change in the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Related references: PMID:28666995
• Mediates cell survival during the DNA damage process through activation of CDK2. Related references: PMID:12839962
• Subcellularly localized to the nucleus.
• Highly expressed in testis, with lower expression in a wide range of tissues including bone marrow, brain, heart, kidney, colon, liver, placenta, spleen, skeletal muscle, and others; expression is cell cycle-dependent and restricted to the G1/S phase.
• Subject to alternative splicing and phosphorylation (phosphoprotein), suggesting the existence of multiple isoforms and post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the full-length human SPDYA (amino acids 1-313), enabling recognition of both native and denatured protein forms.
• For Western blot, expect a band around 36 kDa; optimize gel percentage and transfer conditions accordingly.
• Cross-reactivity with mouse and rat SPDYA is noted; verify performance in your specific sample system.
• For ELISA, consider optimizing coating buffer and antibody dilution; both direct and indirect ELISA protocols can be applied.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents tailored for cell cycle and cancer diagnostic research. This anti-SPDYA antibody is part of our one-stop sourcing portfolio, supporting manufacturers and laboratories from early development to clinical application. Contact us for bulk pricing and customized solutions.