Rabbit anti-SHP/NR0B2 Polyclonal Antibody for WB and ELISA - Q15466

Core Product Specifications and Parameters

Biological Background: SHP/NR0B2 Function and Localization

• SHP/NR0B2 (Small Heterodimer Partner) is an orphan nuclear receptor encoded by the NR0B2 gene; it functions primarily as a transcriptional repressor.
• It inhibits the transactivation activity of various nuclear receptors, serving as a negative regulator of receptor-dependent signaling pathways. Related references: PMID:22504882
• SHP/NR0B2 interferes with NEUROD1-mediated transcription by disrupting the coactivation function of the p300/CBP transcription complex. Related references: PMID:14752053
• As a key component of the liver circadian clock, it interacts with NR1D1 and RORG to regulate NPAS2-mediated hepatic lipid metabolism (By similarity).
• It also regulates the circadian expression of cytochrome P450 (CYP) enzymes, impacting drug metabolism (By similarity).
• The protein localizes to both the nucleus and cytoplasm.
• Expression is predominantly in the liver, with low levels detected in heart and pancreas.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is 28 kDa; validate the actual band migration using appropriate positive controls.
• The immunogen is a recombinant fragment spanning amino acids 40-124, a conserved region in mouse and rat; verify cross-reactivity in your sample system if working with these species.
• In ELISA applications, consider using recombinant SHP/NR0B2 protein or cell lysates; confirm specificity against related nuclear receptors to avoid off-target signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with reliable, one-stop access to IVD raw materials including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. For SHP/NR0B2—a key regulator of nuclear receptor signaling and metabolic pathways—CamelBio can support your projects with optimized polyclonal antibodies and complementary reagents to accelerate assay development from concept to clinic.