Rabbit Anti-RPS6KA6 Polyclonal Antibody for WB, ELISA - Q9UK32

Core Product Specifications and Parameters

Biological Background: RPS6KA6 Function and Localization

• RPS6KA6, also known as RSK4 or p90RSK6, is a member of the ribosomal S6 kinase family and a constitutively active serine/threonine-protein kinase.
• It exhibits growth-factor-independent kinase activity and participates in p53/TP53-dependent cell growth arrest signaling, potentially playing an inhibitory role during embryogenesis.
• The protein localizes to the cytoplasm, cytosol, and nucleus, enabling diverse signaling functions.
• RPS6KA6 belongs to the AGC kinase family and contains two non-identical kinase domains, characteristic of the RSK subfamily.
• It is subject to alternative splicing and is a phosphoprotein, with documented ATP-binding and metal-binding properties essential for its catalytic activity.
• The human RPS6KA6 gene (Gene ID: 27330) encodes a protein with a predicted molecular weight of approximately 84 kDa (UniProt: 83.9 kDa).

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 646-745 of human RPS6KA6) targets the C-terminal region, which may help distinguish this kinase from closely related RSK family members; confirm specificity in your model system.
• For Western blot analysis, a band near 84 kDa is anticipated; validate using appropriate human or mouse cell lysates and include blocking and wash optimization steps.
• ELISA applications can be developed using recombinant RPS6KA6 protein or peptide to establish assay sensitivity and specificity; cross-reactivity with mouse samples should be verified under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for kinase research and IVD development. Whether you require antibodies against RPS6KA6 or other rare kinase targets, CamelBio streamlines procurement from concept to clinic, backed by technical consultation and supply chain reliability.