Rabbit anti-Rat IFN-gamma Monoclonal Antibody () for Flow Cytometry - P01581

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• IFN-gamma is a type II interferon, a cytokine critical for innate and adaptive immunity against viral, bacterial, and protozoal infections.
• It is produced predominantly by activated T lymphocytes and natural killer (NK) cells.
• Signaling through the IFN-gamma receptor (IFNGR1/IFNGR2) activates the JAK-STAT pathway, leading to transcription of interferon-stimulated genes.
• Plays a key role in antigen presentation by inducing immunoproteasome subunits and upregulating MHC class I and II molecules on the cell surface.
• Involved in the regulation of hematopoietic stem cell quiescence and differentiation under homeostatic conditions.
• Post-translational modifications include glycosylation and N-terminal pyroglutamate formation (Pyrrolidone carboxylic acid).
• Secreted protein, released primarily from activated immune cells.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, fixation and permeabilization steps should be optimized for your specific cell type. Standard protocols using paraformaldehyde fixation and saponin-based permeabilization may be suitable.
• Include unstimulated and stimulated rat T cell controls to confirm antibody specificity. PMA/ionomycin stimulation of rat splenocytes is a common positive control.
• The conjugate is excited by 633-647 nm lasers and emits in the far-red channel (e.g., APC channel). Ensure appropriate compensation settings.
• The immunogen spans amino acids 20-156, corresponding to the mature IFN-gamma protein, suggesting the antibody recognizes the natural secreted form.
• Titrate the antibody concentration for optimal staining and minimal background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-rat IFN-gamma monoclonal antibody supports cytokine detection and immune response research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing solutions for your IVD development needs.