Rabbit anti-Rat ICAM-1/CD54 Monoclonal Antibody for Flow Cytometry - Q00238

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (Intercellular adhesion molecule 1), also known as CD54, is a cell surface glycoprotein belonging to the immunoglobulin superfamily.
• It functions as a ligand for the leukocyte integrins LFA-1 (ITGAL:ITGB2) and Mac-1 (ITGAM:ITGB2), mediating leukocyte adhesion and trans-endothelial migration.
• ICAM-1 engagement promotes the assembly of endothelial apical cups during leukocyte diapedesis through ARHGEF26/SGEF and RHOG activation.
• It also facilitates cell aggregation in epithelial cells via interaction with MUC1.
• Localized primarily to the cell membrane, ICAM-1 is a transmembrane protein with extracellular immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination (Ubl conjugation), as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen (aa 28-493) corresponds to the entire extracellular domain of rat ICAM-1, suggesting the antibody likely binds to an epitope in this region accessible on live cells.
• This antibody is validated for flow cytometry; consider optimizing the staining concentration and incubation conditions for your specific cell type.
• Include appropriate controls such as isotype-matched negative control and fluorescence-minus-one (FMO) control to confirm specificity.
• For best results, use fresh, viable cells and avoid fixation unless previously determined to preserve the epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you need routine immune reagents or rare-target sourcing, our platform bridges discovery and diagnostic development. This anti-ICAM-1 antibody supports research in leukocyte biology, inflammation, and cell adhesion, and we offer complementary raw materials to accelerate your assay development and manufacturing.