Rabbit anti-Rat ICAM-1/CD54 Monoclonal Antibody for FC - Q00238

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (Intercellular adhesion molecule 1), also known as CD54, is a cell surface glycoprotein encoded by the Icam1 gene in rats.
• It functions as a ligand for the integrin receptors ITGAL:ITGB2 (LFA-1) and ITGAM:ITGB2 (MAC-1), mediating leukocyte cell-cell adhesion and calibrated T-cell killing.
• During leukocyte trans-endothelial migration, ICAM-1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
• ICAM-1 also promotes cell aggregation in epithelial cells through interaction with MUC1.
• The protein contains several immunoglobulin-like domains, classifying it as a member of the immunoglobulin superfamily.
• It is a single-pass transmembrane protein localized at the cell membrane.
• Post-translational modifications include disulfide bonds, glycosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 28–493 of rat ICAM-1 (extracellular domain), suggesting the antibody likely recognizes an epitope in the extracellular region.
• For flow cytometry, titrate the antibody to determine the optimal working concentration for your cell type and staining conditions.
• Use appropriate isotype controls and viability dyes to ensure specific staining.
• As this antibody is directly conjugated to , ensure it is compatible with your instrument’s laser and filter configuration; carefully plan multicolor panels to avoid spectral overlap.
• Confirm cross-reactivity with your sample; this antibody has been raised against rat ICAM-1 and is specified for rat reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering a seamless sourcing experience for diagnostic developers worldwide. This anti-ICAM-1/CD54 monoclonal antibody reflects our commitment to delivering high-quality reagents for immunology and cell adhesion research. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can assist with custom sourcing for rare or specialized targets, supporting your project from initial development through to clinical application.