Rabbit anti-Rat CD4 Monoclonal Antibody for Flow Cytometry - P05540

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4, also known as T-cell surface antigen T4/Leu-3 and W3/25 antigen in rat, is a cell surface glycoprotein and a member of the immunoglobulin superfamily.
• Functions as a coreceptor for MHC class II molecules on antigen-presenting cells, facilitating T-cell receptor (TCR) signaling by recruiting the tyrosine kinase LCK to the TCR-CD3 complex.
• Essential for thymic development of T-helper cells and triggers differentiation of monocytes into mature macrophages.
• In macrophages and natural killer (NK) cells, CD4 contributes to differentiation, activation, cytokine expression, and cell migration via TCR/LCK-independent pathways.
• Encoded by the Cd4 gene in rat (Gene ID: 24933; human ortholog Gene ID: 920).
• Localized to the cell membrane as a single-pass type I membrane protein.
• Post-translational modifications include disulfide bond formation, N-glycosylation, and palmitoylation, which regulate its membrane localization and function.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant protein spanning amino acids 1-394 of rat CD4, which covers most of the extracellular domain, making it suitable for detecting surface CD4 by flow cytometry.
• For flow cytometry experiments, titrate the antibody to determine the optimal staining concentration for your cell type to minimize nonspecific binding and achieve clear population separation.
• Use appropriate isotype controls and fluorescence-minus-one (FMO) controls when setting up multicolor panels to ensure accurate gating, especially with the bright dye.
• Significant CD4 expression is expected in rat T-helper cells, monocytes, macrophages, and subsets of NK cells; validate performance in these populations using biologically relevant positive and negative controls.
• Because this is a monoclonal antibody, lot-to-lot consistency is generally high; however, each new lot should be validated in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement. This Rabbit anti-Rat CD4 mAb supports immunology and T-cell biology research, and CamelBio can assist with integrating it into diagnostic workflows or scaling up for assay development.